effect of different topical agents on remineralization of early enamel lesion– an in vitro study

objective: this study aimed to compare the effect of using casein phosphopeptide – amorphous calcium phosphate (cpp-acp) paste, remin-pro and fluoride varnish on remineralization of enamel lesions. methods: in this experimental-in vitro study, 60 intact premolars and molars were used and flat enamel surfaces were prepared. the specimens were divided into 6 groups (n=10). after primary diagnodent value measurement and a four-day immersion in demineralizing solution, the diagnodent value were measured. groups 1, 2 and 3 were treated by fluoride varnish, cpp-acp and remin-pro respectively, according to the manufacturer instruction and their diagnodent value was read. groups 4, 5, and 6 were treated by fluoride varnish, cpp-acp and remin-pro for 1 month (8 hours a day), respectively, and their diagnodent value was measured. then the specimens of these three groups were demineralized and ph cycled and their diagnodent values were recorded. the data were analyzed by one-way analysis of variance (anova) and repeated measures anova. results: after a one-month treatment, the diagnodent value significantly decreased in groups 4, 5, and 6 in comparison to the manufacturer instruction (p<0.001). anova test indicated that decrease mean value of diagnodent value was significantly higher for remin-pro and cpp-acp groups than fluoride varnish group, from entrance time to the study to re-demineralization stage (p<0.001). conclusion: all the three materials showed a statistically significant amount of remineralization after repeated application but the cpp-acp and remin pro were more resistant to redemineralization and ph cycling.